Agent skill

qiime2-microbiome-analyzer

QIIME2 microbiome analysis skill for 16S rRNA profiling and diversity analysis

Stars 514
Forks 31

Install this agent skill to your Project

npx add-skill https://github.com/a5c-ai/babysitter/tree/main/library/specializations/domains/science/bioinformatics/skills/qiime2-microbiome-analyzer

Metadata

Additional technical details for this skill

tags
metagenomics microbiome 16s diversity
version
1.0
category
bioinformatics

SKILL.md

QIIME2 Microbiome Analyzer Skill

Purpose

Enable QIIME2 microbiome analysis for 16S rRNA profiling and diversity analysis.

Capabilities

  • Demultiplexing and denoising (DADA2)
  • Taxonomic classification
  • Alpha diversity metrics
  • Beta diversity analysis
  • Differential abundance testing
  • Phylogenetic analysis

Usage Guidelines

  • Use DADA2 for denoising amplicon data
  • Select appropriate taxonomic classifier
  • Calculate diversity metrics with rarefaction
  • Visualize beta diversity with ordination
  • Test differential abundance appropriately
  • Document pipeline parameters

Dependencies

  • QIIME2
  • DADA2
  • phyloseq

Process Integration

  • 16S rRNA Microbiome Analysis (16s-microbiome-analysis)

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