Primer Design Check

In silico primer validation tool.

Use Cases

• qPCR primer design
• Sequencing primer check
• Mutagenesis primer validation

Parameters

• forward_primer: F sequence
• reverse_primer: R sequence
• template: Target genome (optional)

Returns

• Dimer prediction
• Hairpin analysis
• Off-target BLAST results
• Tm and GC% calculations

Example

Flags: Self-dimer detected at 3' end → redesign recommended

Risk Assessment

Security Checklist

• No hardcoded credentials or API keys
• No unauthorized file system access (../)
• Output does not expose sensitive information
• Prompt injection protections in place
• Input file paths validated (no ../ traversal)
• Output directory restricted to workspace
• Script execution in sandboxed environment
• Error messages sanitized (no stack traces exposed)
• Dependencies audited

Prerequisites

No additional Python packages required.

Evaluation Criteria

Success Metrics

• Successfully executes main functionality
• Output meets quality standards
• Handles edge cases gracefully
• Performance is acceptable

Test Cases

1. Basic Functionality: Standard input → Expected output
2. Edge Case: Invalid input → Graceful error handling
3. Performance: Large dataset → Acceptable processing time

Lifecycle Status

• Current Stage: Draft
• Next Review Date: 2026-03-06
• Known Issues: None
• Planned Improvements:
  • Performance optimization
  • Additional feature support