Agent skill

nextflow-pipelines-generate-automatically

Sub-skill of nextflow-pipelines: Generate automatically (+2).

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Install this agent skill to your Project

npx add-skill https://github.com/vamseeachanta/workspace-hub/tree/main/.claude/skills/_archive/science/bio-research/nextflow-pipelines/generate-automatically

SKILL.md

Generate automatically (+2)

Generate automatically

bash
python scripts/generate_samplesheet.py /path/to/data <pipeline> -o samplesheet.csv

The script:

  • Discovers FASTQ/BAM/CRAM files
  • Pairs R1/R2 reads
  • Infers sample metadata
  • Validates before writing

For sarek: Script prompts for tumor/normal status if not auto-detected.

Validate existing samplesheet

bash
python scripts/generate_samplesheet.py --validate samplesheet.csv <pipeline>

Samplesheet formats

rnaseq:

csv
sample,fastq_1,fastq_2,strandedness
SAMPLE1,/abs/path/R1.fq.gz,/abs/path/R2.fq.gz,auto

sarek:

csv
patient,sample,lane,fastq_1,fastq_2,status
patient1,tumor,L001,/abs/path/tumor_R1.fq.gz,/abs/path/tumor_R2.fq.gz,1

*See sub-skills for full details.*

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